The histology research lab is equipped to run ACD’s RNAscope using the Leica Bond RX automated detection system.
How Spatial Genomic ISH Works
Leading the field in spatial genomics, RNAscope Technology is a novel in situ hybridization (ISH) assay for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH approaches with its proprietary probe design to amplify target-specific signals but not background noise from non-specific hybridization.
To find out more, please visit the ACD website.
ACD sells distinctive detection kits for each platform that are not interchangeable, please make sure you order the correct detection kit.
Automated detection singleplex kit for Leica Bond RX
RNAscope 2.5 LSx Reagent Kit RED. Order number 322750
Automated detection duplex kit for Leica Bond RX
RNAscope 2.5 LS Duplex Reagent Kit. Order number 322440
We can run both singleplex and duplex assays. Researchers are responsible for ordering correct detections kits through ACD and acquiring the probes of interest that correspond to the experiment desired.
Catalog Probes and New Probe Designs
Probes ordered have to be compatible with our detection system. If doing singleplex, probes must be for Leica singleplex assays. Alternatively, if ordering for duplex, probes must be ordered for Leica duplex assays. These probes are not interchangeable and cannot be used on different platform systems, including manual detection. YOU MUST be specific when ordering a probe and make sure that you are ordering it for the correct platform and detection system.
Catalog probes
New probe designs
ISH RNAscope Pricing
Pricing for each detection type is priced per slide, and it’s up to the researcher to assess how many slides will be needed to complete the desired experiment. Please consider control slides, optimization slides and any repeats into your calculations.
The singleplex kit from ACD includes the red chromogen detection and is “ready to use.” Therefore, this is reflected in the kit cost and in our per-slide price. The duplex kit from ACD does not include chromogen detection. We are providing the chromogen detection for both channels, red and green. The kit does not include titer containers. We are providing these to the researcher. The price per slide reflects the additional supplies required for duplex runs.
Singleplex: $55.90 per slide
Duplex: $151.80 per slide
