Histology Lab

Processing, Embedding, Sectioning, Services

Block, Slide, H&E

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Block, Slide Special stain

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Process and Embed only

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Process Block, Custom Special

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Unstained slide

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Re-cut/H&E In house

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H&E on Smear

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Additional Levels Per Block

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Cover slipping only

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Scrolls for PCR (6-10micron)

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Special Stain-Slide

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Slide Special on unstained slide

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Special Stain-Custom

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Sm. Eye Processing

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Lg Eye Processing

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Special Stains

Acid-Fast Bacteria (Ziehl-Neelsen Stains)

AFB

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for Identification of Mycobacterium.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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GMS

GMS

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of urates in tissue.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Gram

Gram

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of Gram -/+ bacteria.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Periodic Acid-Schiff (PAS)

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: The demonstration of polysaccharides, neutral mucosubstances, and basement membranes. 
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Giemsa

Geisma

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the detection of H pylori in tissue.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Toluidine Blue

T Blue

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of mast cells in tissue. 
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Acid-Fast Bacteria (Kinyouns Stain)

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for Identification of Mycobacterium
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Melanin Bleach

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: When melanin pigment is present in large amounts cell detail may be obscured. Excess melanin can be removed from tissue sections.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Periodic Acid-Schiff (PAS) Fungus

Days tested: Monday-Friday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Demonstration of fungi in tissue.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Bile

Bile

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of bilirubin and bile pigments in tissue.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Congo Red

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of amyloid in tissues.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Copper

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the detection of copper in tissue, especially in liver in Wilson disease.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Fontana-Masson

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of argentaffin substances.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
Test codes: Tuesdays and Thursdays

Iron

Iron

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Detects the ferric ion in loosely bound protein complexes. 
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Trichrome

Trichrome

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used to differentiate collagen from smooth muscle in tumors.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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VonKossa

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used to identify the presence of calcium in tissue.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Wathin-Starry

Days tested: Tuesdays and Thursdays
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of spirochetes in tissue sections.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Alcian Blue

Days tested: Wednesday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstration of acid mucopolysaccharides.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Luxol Fast Blue/PAS

Days tested: Wednesday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used for the demonstation of myelin in tissue.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Periodic Acid-Schiff (PAS) with and without Diastase

Days tested: Wednesday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: The demonstration of glycogen in tissue sections.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Mucicarmine Mayer

Days tested: Wednesday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Staining of “epithelial” mucin in tissue sections. Also demonstrates C neoformans.
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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PTAH

Days tested: Wednesday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Demonstration of muscle cross-striations and fibrin. Can also be used for glial fibers and myelin. 
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Verhoff Elastic

Days tested: Wednesday
Specimen requirements: Tissue, Paraffin Block or Unstained Slide
Stain information: Used to demonstrate pathological changes in elastic fibers. 
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Estimated turnaround time: The turn around time for all stains is between 1-2 days (for staining). Interpretation (as part of necropsy or biopsy cases) will take an extra 1-3 days.
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Immunohistochemistry

IHC (antibody supplied)

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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Ckit (CD117)

ckit

Days tested: Monday-Friday
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Stain information: Recognizes the extracellular domain and is expressed by normal & abnormal cell types. (Normal: breast epithelium, germ, melanoctyes, stem, mast. Abnormal: testicular germ, endometrial carcinomas, melanomas, ovarian epi. Carcinomas)
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Estimated turnaround time: 1 week
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CD79

CD79

Days tested: Monday-Friday
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Stain information: B-cell Receptor, precursor-B-cell
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Estimated turnaround time: 1 week
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Iba1

Iba1

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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Vimentin

Vimentin

Days tested: Monday-Friday
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Stain information: Main filament protein in mesenchymal cells. Diagnostic of melanoma and sarcoma.
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Estimated turnaround time: 1 week
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CD3

CD3

Days tested: Monday-Friday
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Stain information: Expressed by T cells
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Estimated turnaround time: 1 week
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Desmin

Desmin

Days tested: Monday-Friday
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Stain information: Reacts with striated & smooth muscle cells. (Z-bands)
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MSA

MSA

Days tested: Monday-Friday
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Stain information: Recognizes the alpha actin from cardiac, skeletal and smooth muscle sources
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Estimated turnaround time: 1 week
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SMA

SMA

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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FIP

FIP

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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Factor 8

F8

Days tested: Monday-Friday
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Stain information: Endothelial cells (cytoplasmic staining)
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Estimated turnaround time: 1 week
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Glucagon

Glucagon

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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Insulin

Insulin

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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Synaptophysin

Synaptophysin

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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Mum 1

Days tested: Monday-Friday
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Stain information: Expressed in the nuclei and cytoplasm of plasma cells and a small percentage of germinal center B cells
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Estimated turnaround time: 1 week
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Chromogranin

Chromogranin

Days tested: Monday-Friday
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Stain information: Expressed by neuroendocrine cells
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Estimated turnaround time: 1 week
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Cytokeratin

Cytokeratin

Days tested: Monday-Friday
Specimen requirements:
Stain information: Identification of a broad spectrum of acidic and basic cytokeratin proteins.
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Estimated turnaround time: 1 week
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GFAP

GFAP

Days tested: Monday-Friday
Specimen requirements:
Stain information: Schwann, enteric glial, & satellite cells (weak staining: axons observed) Distinguishes neoplasms vs. neoplasms of astrocytic origin
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Estimated turnaround time: 1 week
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NSE

NSE

Days tested: Monday-Friday
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Estimated turnaround time: 1 week
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PNL2

PNL2 on Melanoma

Days tested: Monday-Friday
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Stain information: Yields a strong cytoplasmic staining of skin and oral mucosal melanocytes
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Estimated turnaround time: 1 week
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TTF1

TTF1

Days tested: Monday-Friday
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Stain information: Expressed in epithelial cells of the thyroid gland and the lung.
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Estimated turnaround time: 1 week
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