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Microbiology

Test

Required Sample

Shipping

Aerobic culture and susceptibility Fluid, tissue, or culturette Ice pack
Anaerobic culture (no susceptibility, sent out for additional testing upon request) Fluid or tissue in anaerobic transport system Ice pack
Bacterial Identification by 16s PCR Sterile site or bacterial isolate  Ice pack
Blood Culture Blood culture bottle system Ambient
Clostridium difficile toxin A/B ELISA Fresh feces Ice pack
Clostridium perfringens culture 0.5 g feces or swab Ice pack
Clostridium perfringens enterotoxin ELISA Fresh feces Ice pack
Dermatophyte culture Hair, skin scraping, or culture Ambient
E. coli O157:H7 0.5 g feces or swab Ice pack
Fungal culture Fluid, tissue (preferred over culturette) Ice pack
Fungal Susceptibility Panel Added to culture order  —
Gram Stain Fluid, tissue, smear Ice pack
Mycobacterium culture Fluid, tissue (preferred over culturette) Ice pack
Mycobacterium/ Aerobic Actinomycete Susceptibility Panel Added to culture order
Mycoplasma culture (no susceptibility)
Reptile testing available
Fluid or swab in Mycoplasma transport medium
(if medium needed call 352-294-4726)
Ice pack
Penicillin Binding Protein 2 Agglutination Added to culture order
Pythium culture (no susceptibility) Canine: lesion tissue
Equine: kunker material
Ice pack
Rotavirus antigen ELISA 0.5 g feces Ice pack
Salmonella culture (no susceptibility) 1 g feces Ice pack
Salmonella susceptibility and serogroup Add to culture or provide isolate Ambient
Shigella culture 0.5 g feces/swab Ice pack
Yersinia culture 0.5 g feces/swab Ice pack

Handling and shipping advice for microbiology samples

  • To reduce bacterial overgrowth, send most samples overnight with an ice pack (exception: blood culture bottles and cultures for trichmonads).
  • Tissue or liquid in a sterile, leak-proof container enhances recovery of microorganisms.
  • Culturettes are acceptable, but tissue or fluid is preferred. Please submit one culturette per culture request if possible (example, 3 swabs if aerobic, anaerobic, and fungal are requested).
  • Tissue is highly recommended for successful recovery of fungus, mycobacterium, or pythium.
  • Anaerobic culture samples need to be submitted using an anaerobic transport system (Amies Gel or anaerobic vial/bag)
  • Blood cultures need to be submitted in blood culture bottles and kept at room temperature.
  • Include a completed a microbiology, parasitology & serology submission form with billing information, patient information, sample source(s), pertinent history and/or provisional diagnosis, and desired test(s).
  • The required sample type and shipping method for each test are listed above.